In vitro Sperm Function Tests and Testicular Biometry for Fertility Prediction in Boar

Amle, M. B., Mundhe, S.M., Birade H.S.

Abstract


The study was conducted on six porcine males and thirty six semen ejaculates for its fertility assessment. The average length of the left and right testicle of the boars measured by ultrasonography was 9.47 ± 0.73 cm and 9.09 ± 0.65 cm, respectively. The sperm concentration/ml increased significantly as testicular diameter increased in size. The average length of right and left of boar testicle measured by Vernier caliper was 10.4 ± 0.57 cm and average width was 4.3 ± 0.14 cm. The average volume of boar semen was 115.00 ± 11.83 ml with milky colour. Thick consistency was observed in 83.3% semen samples whereas 16.6 % semen samples were having thin consistency. Out of 36 semen ejaculates, 16.6 % semen samples had density of DD where as 83.3 % had a density of DDD. The Mass activity, live percentage, percentage of morphologically abnormal spermatozoa and total sperm concentration in boar semen were 3 ± 0, 75.41 ± 2.07 %, 0 %, and 523 ± 60.07 million/ml, respectively. Mean percentage of hypo osmotic swelling test (HOS-Test) of semen found in the present study was 73.47 ± 2.26. The average time for reduction of resazurin dye from blue to violet was 1.805 ± 0.163 and from violet to pink was 9.944 ± 0.890. None of the sample change colour from pink to white. In the present study 36 ejaculates of boar semen were subjected for TVC of bacteria. In order to differentiate the bacterial species contaminating semen raw semen was placed on different agar plates. The species isolated with higher frequency in boar semen were of Staphylococcus species-83.3% (30 samples) followed by E.coli -63.8% (23 samples). Mean TVC obtained in this study was 45.13 × 103.


Keywords


Porcine; semen; sperm function tests; testicular biometry

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