Effect of Stocking Density Stress on the Hematological Profile of Oncorhynchus mykiss

Abstract

The present research aimed at the investigation into the effect of stocking density on the hematological response of Oncorhynchus mykiss maintained in flow through condition. The stock having a weight of 520.22 ± 48.20 g and 580.25 ± 52.2 g were stocked in flow through FRP tanks at the stocking density of 38 kg/m³ and 30 kg/m³ respectively. In order to collect blood sample for analysis, the blood was collected through cardiac puncture. Red blood cell counts (RBCc), hematocrit values (Hct), hemoglobin concentration (Hb), were analyzed through haemoanalyser, while as mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were calculated through standard formulas. Differences in hematological parameters were statistically analyzed by Student’s T-Test. The stocking density stress was evident from the significant increase (p<0.05) in RBC, Hb, Ht, MCV and insignificant decrease (p>0.05) in MCH and MCHC.