Extraction of Genomic DNA from Gossypium sps. Without Detergent Suitable for PCR
| Dublin Core | PKP Metadata Items | Metadata for this Document | |
| 1. | Title | Title of document | Extraction of Genomic DNA from Gossypium sps. Without Detergent Suitable for PCR |
| 2. | Creator | Author's name, affiliation, country | M. Jayasravanthi; Department of Biotechnology, Acharya Nagarjuna University, Guntur, Andhra Pradesh; India |
| 2. | Creator | Author's name, affiliation, country | A. Krishnasatya; Department of Biotechnology, Acharya Nagarjuna University, Guntur, Andhra Pradesh; India |
| 2. | Creator | Author's name, affiliation, country | P. Chandrasekhar; Division of R&D, Tulasi Seeds Pvt. Ltd., Guntur, Andhra Pradesh; India |
| 3. | Subject | Discipline(s) | BIOTECHNOLOGY; MOLECULAR BIOLOGY |
| 3. | Subject | Keyword(s) | Cotton, DNA, SDS, Microsatellites |
| 4. | Description | Abstract |
Extraction of DNA is the prerequisite for many biotechnological works like marker assisted selection particularly in commercial crops such as cotton. Here we report a simple, rapid and inexpensive procedure for the isolation of DNA from cotton seeds for laboratory purpose. This procedure avoids the usage of detergents like CTAB/SDS which are inconvenient in preparation and time consuming. In addition, this procedure involves the usage of various reagents in different combinations for the effective removal of polyphenols, proteins, salts and polysaccharides making DNA suitable for PCR. Therefore, this method can be employed for various applications even in low equipped laboratories. |
| 5. | Publisher | Organizing agency, location | |
| 6. | Contributor | Sponsor(s) | TULASI SEEDS PRIVATE LIMITED |
| 7. | Date | (YYYY-MM-DD) | 2012-11-19 |
| 8. | Type | Status & genre | Peer-reviewed Article |
| 8. | Type | Type | |
| 9. | Format | File format | |
| 10. | Identifier | Uniform Resource Identifier | http://scientific.cloud-journals.com/index.php/IJAAST/article/view/Sci-23 |
| 11. | Source | Journal/conference title; vol., no. (year) | International Journal of Advanced Agricultural Science and Technology; Vol 1, No 1 (2012) |
| 12. | Language | English=en | en |
| 14. | Coverage | Geo-spatial location, chronological period, research sample (gender, age, etc.) | |
| 15. | Rights | Copyright and permissions |
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