Indexing metadata

Molecular Characterization of Sperm Antigens with In Vivo Developed Antisperm Antibodies in Variably Inseminated Cross Bred-Heifers/Cows


 
Dublin Core PKP Metadata Items Metadata for this Document
 
1. Title Title of document Molecular Characterization of Sperm Antigens with In Vivo Developed Antisperm Antibodies in Variably Inseminated Cross Bred-Heifers/Cows
 
2. Creator Author's name, affiliation, country Ranjna S. Cheema; Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
 
2. Creator Author's name, affiliation, country Amrit K. Bansal; Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
 
3. Subject Discipline(s) Reproductive Physiology
 
3. Subject Keyword(s) Sperm; Antigens; ASA; Heifers; Cows
 
3. Subject Subject classification Reproductive Immunology
 
4. Description Abstract

Molecular characterization of sperm proteins and their immunoreactions to sera from subfertile/infertile animals may give deeper insight to the immunological reason of infertility. The purpose of this study was to characterize antigenic sperm proteins by reacting with blood serum and cervical mucus of variably inseminated heifers/cows using immunoblotting and immunofluorescence techniques. Immunoblot analysis of sperm proteins with blood serum/CM of variably inseminated cows/heifers revealed around 23 polypeptides in cattle sperm, but all proteins did not react with blood serum and cervical mucus of all tested animals. Number of sperm polypeptides that reacted with blood serum of non-inseminated, 1-3, 4-6 and >6 times inseminated heifers/cows were in the range of 11-14, 8-13, 3-14 and 5-13 respectively. Correspondingly cervical mucus of 1-3, 4-6 and >6 times inseminated heifers/cows also recognized about 2-7, 2-13 and 2-12 sperm polypeptides respectively. Immunofluorescence of sperm smears with blood serum and cervical mucus of variably inseminated heifer/cows indicated the presence of antisperm antibodies (ASA) mainly against acrosome surface, post –acrosome and principal piece surface proteins. There was not any difference in range of number of polypeptides detected with blood serum in regard to number of insemination. But range of number of polypeptides detected was higher with cervical mucus of >3 times inseminated animals, which indicated increase in ASA against antigenic sperm proteins in cervical mucus with increase in number of inseminations. It was concluded that ASA are produced in blood serum and cervical mucus of cows/heifers against sperm proteins, irrespective of number of inseminations. In future further characterization of sperm proteins and their homogeneity with bacterial proteins is needed to understand the mechanism of ASA mediated fertility impairment and to develop treatment protocol on the basis of purified antigens.
 
5. Publisher Organizing agency, location
 
6. Contributor Sponsor(s) UGC, New Delhi
 
7. Date (YYYY-MM-DD) 2015-08-22
 
8. Type Status & genre Peer-reviewed Article
 
8. Type Type Approach
 
9. Format File format PDF
 
10. Identifier Uniform Resource Identifier http://scientific.cloud-journals.com/index.php/IJAVST/article/view/Sci-296
11. Source Journal/conference title; vol., no. (year) International Journal of Advanced Veterinary Science and Technology; Volume 4 (Year 2015)
 
12. Language English=en en
 
14. Coverage Geo-spatial location, chronological period, research sample (gender, age, etc.) Blood and Cervical mucus
 
15. Rights Copyright and permissions

Copyright Terms & Conditions

Authors who publish with this journal agree to the following terms:

a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work

Cloud Publications reserves the right to amend/change the copyright policy; with/without notice.