Indexing metadata

Complete Genome Characteristics of Porcine circovirus Type 2 (PCV2) Isolates from Papuan Pigs, Indonesia


 
Dublin Core PKP Metadata Items Metadata for this Document
 
1. Title Title of document Complete Genome Characteristics of Porcine circovirus Type 2 (PCV2) Isolates from Papuan Pigs, Indonesia
 
2. Creator Author's name, affiliation, country Widi Nugroho; School of Animal and Veterinary Sciences, Roseworthy Campus, University of Adelaide, Roseworthy, Australia
 
2. Creator Author's name, affiliation, country Farhid Hemmatzadeh; School of Animal and Veterinary Sciences, Roseworthy Campus, University of Adelaide, Roseworthy, Australia
 
2. Creator Author's name, affiliation, country Sidna Artanto; Faculty of Veterinary Medicine, Gadjah Mada University, Jl. Olahraga, Karangmalang, Yogyakarta, Indonesia
 
2. Creator Author's name, affiliation, country Michael Philipp Reichel; School of Animal and Veterinary Sciences, Roseworthy Campus, University of Adelaide, Roseworthy, Australia
 
3. Subject Discipline(s) Molecular epidemiology
 
3. Subject Keyword(s) Papua; Pigs; ORF2; Neighbour Joining; PCV2-IM3 Genotype
 
3. Subject Subject classification Veterinary virology
 
4. Description Abstract

Porcine circovirus type 2 (PCV2) has been recognised as an important pathogen in the pig industry world-wide. The virus was recently identified in Papuan pigs, yet information about the characteristics of Papuan PCV2 isolates is limited. The aim of the present study was to characterise the complete genome of Papuan PCV2 isolates. Viral DNA was isolated from 13 blood samples of village pigs from the Jayawijaya region. Four of the PCR positive samples were selected for full genome sequencing. Neighbour Joining phylogeny with P-distance model showed that the four Papuan PCV2 isolates belong to genotype PCV2-IM3. Sequence identity analysis of the Papuan PCV2 genomes further showed 99.5% to 99.6% similarity with a Chinese PCV2-IM3 reference strain. The current study revealed that genotyping based on the ORF2 sequence resulted in a substantially different characterisation of PCV2 genotypes compared to classification based on the complete genome sequences. Furthermore, the study showed that the topology of the PCV2 phylogeny based on the ORF2 sequence was different from the topology based on capsid proteins. Genotyping using the complete genome, ORF2 region or capsid protein sequences resulted in substantial variance in the classification of the PCV2 isolates. The clinical consequence of these different genotyping methods needs further study.
 
5. Publisher Organizing agency, location
 
6. Contributor Sponsor(s) ACIAR; The University of Adelaide
 
7. Date (YYYY-MM-DD) 2016-04-21
 
8. Type Status & genre Peer-reviewed Article
 
8. Type Type Bioinformatic
 
9. Format File format PDF
 
10. Identifier Uniform Resource Identifier http://scientific.cloud-journals.com/index.php/IJAVST/article/view/Sci-377
11. Source Journal/conference title; vol., no. (year) International Journal of Advanced Veterinary Science and Technology; Volume 5 (Year 2016)
 
12. Language English=en en
 
14. Coverage Geo-spatial location, chronological period, research sample (gender, age, etc.) Papua, Indonesia,
Isolates
 
15. Rights Copyright and permissions

Copyright Terms & Conditions

Authors who publish with this journal agree to the following terms:

a. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

b. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

c. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work

Cloud Publications reserves the right to amend/change the copyright policy; with/without notice.