The effect of breed and sex on some hematological and serum biochemical parameters were studied in twenty two clinically healthy Labrador (eleven numbers each from male and female) and equivalent number of Spitz. Blood samples were estimated for hematological [hemoglobin (Hb), white blood cell count (WBC), packed cell volume (PCV)] and serum biochemical [total protein, albumin, urea, creatinine, alkaline phosphatase (ALKP), aspartate amino transferase (AST), alanine amino transferase (ALT)] parameters. Serum urea was significantly (p<0.05) higher in the male Labrador than in the female where as serum alkaline phosphatase was noted to be significantly (p<0.05) higher in both male and female Labrador as compared to Spitz breeds of corresponding sexes. However other parameters showed non-significant (p>0.05) variation between male and female group of both the breeds.