A study was conducted on bovine bulls in two bio-secure farms in India using Delayed Type of Hypersensitivity (DTH) to determine the prevalence of bovine paratuberculosis or Johne’s disease (JD). The DTH was carried out on the neck of the animals, conducted as per OIE Terrestrial manual 2008. The results of DTH carried out for JD in two farms in western and northern India for a period of five years is being described. Prevalence of JD was found in Jun-10 (0.62%), Oct-10 (0.52%), Dec-10 (0.51%) and May-15 (1.09%) in farm at northern India whereas in western India farm, in Aug-10, May-11 and Mar-12, JD incidence rate was 0.34%, 1.86% and 0.54% respectively. It can be concluded from the present study that JD was present in both the herds with low incidence rate of 0-1.86 percent during the five years.