Production and Partial Characterization of Penicillin Amidase Produced by Bacillus megaterium from Temple Puja Wastes

Abstract

Temple puja wastes are released in water bodies or land creating severe environmental pollution and health hazards. The temple wastes extract was used to isolate the Bacillus megaterium for the production of enzyme penicillin amidase as it recorded the largest zone of activity. The result obtained showed that on nutrient agar medium the grown colonies were confirmed by gram staining as Bacillus megaterium in temple wastes. P.amidase activity was determined using DNSA method. Highest yield of enzyme activity by B.megaterium was obtained after 48 hrs. of incubation. The optimum temperature and pH for the enzyme activity was obtained at 60^oC (6.0+ 0.55) and pH 7.0 (2.04 ± 0.49). The results shown that B.megaterium is a good producer of extracellular P.amidase at high temperature which could be an indication of P.amidase produced as thermostable. The study revealed the potential of agricultural wastes’ capability to produce amylase by B.megaterium.