Molecular Characterization of Corynebacterium bovis causing Clinical Mastitis and Increasing Somatic-Cell Count

Langoni H., Guimarães F.F., Salina A., Ribeiro M. G., Baio P.V.P., Ramos J.N., Mota H.F., Vieira V.V., Mattos-Guaraldi A.L.

Abstract


Bovine mastitis remains the most economically important disease affecting dairy cows. Bacteria of the genus Corynebacterium spp., especially Corynebacterium bovis (C. bovis), are frequently isolated from bovine mastitis although there are divergent reports about the role of this pathogen in bovine mastitis. In the present study we report the presence of new C. bovis strains from milk exhibiting increased SCC of 12 cows with subclinical and clinical mastitis. Irregular Gram-positive bacilli isolated in pure cultures underwent conventional biochemical tests which generated indefinite phenotypic profiles (Corynebacterium mastitidis/C. bovis). API-Coryne System 3.0 identified all the milk isolates as C. bovis (code number 4501014; 99.9% confidence level). Phylogenetic analysis based on 16S rRNA and rpoB sequences confirmed the identification of the strains as C. bovis.


Keywords


Corynebacterium bovis; Mastitis; Sequencing of Genes; 16S rRNA; rpoB

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*2016 Journal Impact Factor was established by dividing the number of articles published in 2014 and 2015 with the number of times they are cited in 2016 based on Google Scholar, Google Search and the Microsoft Academic Search. If ‘A’ is the total number of articles published in 2014 and 2015, and ‘B’ is the number of times these articles were cited in indexed publications during 2016 then, journal impact factor = A/B. To know More: (http://en.wikipedia.org/wiki/Impact_factor)