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Molecular Characterization of Corynebacterium bovis causing Clinical Mastitis and Increasing Somatic-Cell Count


 
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1. Title Title of document Molecular Characterization of Corynebacterium bovis causing Clinical Mastitis and Increasing Somatic-Cell Count
 
2. Creator Author's name, affiliation, country Langoni H.; Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, UNESP – Univ. Estadual Paulista “Júlio de Mesquita Filho”, Botucatu, São Paulo State, Brazil
 
2. Creator Author's name, affiliation, country Guimarães F.F.; Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, UNESP – Univ. Estadual Paulista “Júlio de Mesquita Filho”, Botucatu, São Paulo State, Brazil
 
2. Creator Author's name, affiliation, country Salina A.; Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, UNESP – Univ. Estadual Paulista “Júlio de Mesquita Filho”, Botucatu, São Paulo State, Brazil
 
2. Creator Author's name, affiliation, country Ribeiro M. G.; Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, UNESP – Univ. Estadual Paulista “Júlio de Mesquita Filho”, Botucatu, São Paulo State, Brazil
 
2. Creator Author's name, affiliation, country Baio P.V.P.; Laboratory of Diphtheria and Corynebacteria of Clinical Relevance-LDCIC, Collaborating Center for Diphtheria of CGLAB/SVS/MS, School of Medical Sciences, Universidade do Estado do Rio de Janeiro – UERJ, Rio de Janeiro, RJ, Brazil
 
2. Creator Author's name, affiliation, country Ramos J.N.; Laboratory of Diphtheria and Corynebacteria of Clinical Relevance-LDCIC, Collaborating Center for Diphtheria of CGLAB/SVS/MS, School of Medical Sciences, Universidade do Estado do Rio de Janeiro – UERJ, Rio de Janeiro, RJ, Brazil
 
2. Creator Author's name, affiliation, country Mota H.F.; Laboratory of Diphtheria and Corynebacteria of Clinical Relevance-LDCIC, Collaborating Center for Diphtheria of CGLAB/SVS/MS, School of Medical Sciences, Universidade do Estado do Rio de Janeiro – UERJ, Rio de Janeiro, RJ, Brazil
 
2. Creator Author's name, affiliation, country Vieira V.V.; Laboratory of Diphtheria and Corynebacteria of Clinical Relevance-LDCIC, Collaborating Center for Diphtheria of CGLAB/SVS/MS, School of Medical Sciences, Universidade do Estado do Rio de Janeiro – UERJ, Rio de Janeiro, RJ, Brazil
 
2. Creator Author's name, affiliation, country Mattos-Guaraldi A.L.; Laboratory of Diphtheria and Corynebacteria of Clinical Relevance-LDCIC, Collaborating Center for Diphtheria of CGLAB/SVS/MS, School of Medical Sciences, Universidade do Estado do Rio de Janeiro – UERJ, Rio de Janeiro, RJ, Brazil
 
3. Subject Discipline(s)
 
3. Subject Keyword(s) Corynebacterium bovis; Mastitis; Sequencing of Genes; 16S rRNA; rpoB
 
4. Description Abstract

Bovine mastitis remains the most economically important disease affecting dairy cows. Bacteria of the genus Corynebacterium spp., especially Corynebacterium bovis (C. bovis), are frequently isolated from bovine mastitis although there are divergent reports about the role of this pathogen in bovine mastitis. In the present study we report the presence of new C. bovis strains from milk exhibiting increased SCC of 12 cows with subclinical and clinical mastitis. Irregular Gram-positive bacilli isolated in pure cultures underwent conventional biochemical tests which generated indefinite phenotypic profiles (Corynebacterium mastitidis/C. bovis). API-Coryne System 3.0 identified all the milk isolates as C. bovis (code number 4501014; 99.9% confidence level). Phylogenetic analysis based on 16S rRNA and rpoB sequences confirmed the identification of the strains as C. bovis.
 
5. Publisher Organizing agency, location
 
6. Contributor Sponsor(s) Fapesp
 
7. Date (YYYY-MM-DD) 2016-06-10
 
8. Type Status & genre Peer-reviewed Article
 
8. Type Type
 
9. Format File format PDF
 
10. Identifier Uniform Resource Identifier http://scientific.cloud-journals.com/index.php/IJAVST/article/view/Sci-438
11. Source Journal/conference title; vol., no. (year) International Journal of Advanced Veterinary Science and Technology; Volume 5 (Year 2016)
 
12. Language English=en en
 
14. Coverage Geo-spatial location, chronological period, research sample (gender, age, etc.)
 
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